A false note of DNA polymerase iota in the choir of genome caretakers in mammals.
Identifieur interne : 000242 ( Main/Exploration ); précédent : 000241; suivant : 000243A false note of DNA polymerase iota in the choir of genome caretakers in mammals.
Auteurs : L V Gening [Russie] ; A V Makarova ; A M Malashenko ; V Z TarantulSource :
- Biochemistry. Biokhimiia [ 0006-2979 ] ; 2006.
Descripteurs français
- KwdFr :
- Animaux (MeSH), Codon non-sens (composition chimique), Codon non-sens (métabolisme), DNA-directed DNA polymerase (composition chimique), DNA-directed DNA polymerase (génétique), DNA-directed DNA polymerase (métabolisme), Domaine catalytique (MeSH), Encéphale (enzymologie), Guanine (métabolisme), Inhibiteurs de la synthèse d'acide nucléique (MeSH), Instabilité du génome (MeSH), Mâle (MeSH), Réplication de l'ADN (génétique), Souris (MeSH), Souris de lignée BALB C (MeSH), Souris de lignée C3H (MeSH), Souris de lignée C57BL (MeSH), Spécificité d'organe (génétique), Testicule (effets des radiations), Testicule (enzymologie), Thymine (métabolisme).
- MESH :
- composition chimique : Codon non-sens, DNA-directed DNA polymerase.
- effets des radiations : Testicule.
- enzymologie : Encéphale, Testicule.
- génétique : DNA-directed DNA polymerase, Réplication de l'ADN, Spécificité d'organe.
- métabolisme : Codon non-sens, DNA-directed DNA polymerase, Guanine, Thymine.
- Animaux, Domaine catalytique, Inhibiteurs de la synthèse d'acide nucléique, Instabilité du génome, Mâle, Souris, Souris de lignée BALB C, Souris de lignée C3H, Souris de lignée C57BL.
English descriptors
- KwdEn :
- Animals (MeSH), Brain (enzymology), Catalytic Domain (MeSH), Codon, Nonsense (chemistry), Codon, Nonsense (metabolism), DNA Replication (genetics), DNA-Directed DNA Polymerase (chemistry), DNA-Directed DNA Polymerase (genetics), DNA-Directed DNA Polymerase (metabolism), Genomic Instability (MeSH), Guanine (metabolism), Male (MeSH), Mice (MeSH), Mice, Inbred BALB C (MeSH), Mice, Inbred C3H (MeSH), Mice, Inbred C57BL (MeSH), Nucleic Acid Synthesis Inhibitors (MeSH), Organ Specificity (genetics), Testis (enzymology), Testis (radiation effects), Thymine (metabolism).
- MESH :
- chemical , chemistry : Codon, Nonsense, DNA-Directed DNA Polymerase.
- enzymology : Brain, Testis.
- genetics : DNA Replication, DNA-Directed DNA Polymerase, Organ Specificity.
- chemical , metabolism : Codon, Nonsense, DNA-Directed DNA Polymerase, Guanine, Thymine.
- radiation effects : Testis.
- Animals, Catalytic Domain, Genomic Instability, Male, Mice, Mice, Inbred BALB C, Mice, Inbred C3H, Mice, Inbred C57BL, Nucleic Acid Synthesis Inhibitors.
Abstract
DNA polymerase iota (Pol iota) of mammals is a member of the Y family of DNA polymerases. Among many other genome caretakers, these enzymes are responsible for maintaining genome stability. The members of the Y-family DNA polymerases take part in translesion DNA synthesis, bypassing some DNA lesions, and are characterized by low fidelity of DNA synthesis. A unique ability of Pol iota to predominantly incorporate G opposite T allowed us to identify the product of this enzyme among those synthesized by other DNA polymerases. This product can be called a "false note" of Pol iota. We measured the enzyme activity of Pol iota in crude extracts of cells from different organs of five inbred strains of mice (N3H/Sn, 101/H, C57BL/6, BALB/c, 129/J) that differed in a number of parameters. The "false note" of Pol iota was clearly sounding only in the extracts of testis and brain cells from four analyzed strains: N3H/Sn, 101/H, C57BL/6, BALB/c. In mice of 129/J strain that had a nonsense mutation in the second exon of the pol iota gene, the Pol iota activity was reliably detectable only in the extracts of brain. The data show that the active enzyme can be formed in some cell types even if they carry a nonsense mutation in the pol iota gene. This supports tissue-specific regulation of pol iota gene expression through alternative splicing. A semiquantitative determination of pol iota activity in mice strains different in their radiosensitivity suggests a reciprocal correlation between the enzyme activity of pol iota in testis and the resistance of mice to radiation.
DOI: 10.1134/s0006297906020064
PubMed: 16489919
Affiliations:
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Among many other genome caretakers, these enzymes are responsible for maintaining genome stability. The members of the Y-family DNA polymerases take part in translesion DNA synthesis, bypassing some DNA lesions, and are characterized by low fidelity of DNA synthesis. A unique ability of Pol iota to predominantly incorporate G opposite T allowed us to identify the product of this enzyme among those synthesized by other DNA polymerases. This product can be called a "false note" of Pol iota. We measured the enzyme activity of Pol iota in crude extracts of cells from different organs of five inbred strains of mice (N3H/Sn, 101/H, C57BL/6, BALB/c, 129/J) that differed in a number of parameters. The "false note" of Pol iota was clearly sounding only in the extracts of testis and brain cells from four analyzed strains: N3H/Sn, 101/H, C57BL/6, BALB/c. In mice of 129/J strain that had a nonsense mutation in the second exon of the pol iota gene, the Pol iota activity was reliably detectable only in the extracts of brain. The data show that the active enzyme can be formed in some cell types even if they carry a nonsense mutation in the pol iota gene. This supports tissue-specific regulation of pol iota gene expression through alternative splicing. A semiquantitative determination of pol iota activity in mice strains different in their radiosensitivity suggests a reciprocal correlation between the enzyme activity of pol iota in testis and the resistance of mice to radiation.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16489919</PMID><DateCompleted><Year>2006</Year><Month>05</Month><Day>16</Day></DateCompleted><DateRevised><Year>2019</Year><Month>09</Month><Day>17</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0006-2979</ISSN><JournalIssue CitedMedium="Print"><Volume>71</Volume><Issue>2</Issue><PubDate><Year>2006</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Biochemistry. Biokhimiia</Title></Journal><ArticleTitle>A false note of DNA polymerase iota in the choir of genome caretakers in mammals.</ArticleTitle><Pagination><MedlinePgn>155-9</MedlinePgn></Pagination><Abstract><AbstractText>DNA polymerase iota (Pol iota) of mammals is a member of the Y family of DNA polymerases. Among many other genome caretakers, these enzymes are responsible for maintaining genome stability. The members of the Y-family DNA polymerases take part in translesion DNA synthesis, bypassing some DNA lesions, and are characterized by low fidelity of DNA synthesis. A unique ability of Pol iota to predominantly incorporate G opposite T allowed us to identify the product of this enzyme among those synthesized by other DNA polymerases. This product can be called a "false note" of Pol iota. We measured the enzyme activity of Pol iota in crude extracts of cells from different organs of five inbred strains of mice (N3H/Sn, 101/H, C57BL/6, BALB/c, 129/J) that differed in a number of parameters. The "false note" of Pol iota was clearly sounding only in the extracts of testis and brain cells from four analyzed strains: N3H/Sn, 101/H, C57BL/6, BALB/c. In mice of 129/J strain that had a nonsense mutation in the second exon of the pol iota gene, the Pol iota activity was reliably detectable only in the extracts of brain. The data show that the active enzyme can be formed in some cell types even if they carry a nonsense mutation in the pol iota gene. This supports tissue-specific regulation of pol iota gene expression through alternative splicing. A semiquantitative determination of pol iota activity in mice strains different in their radiosensitivity suggests a reciprocal correlation between the enzyme activity of pol iota in testis and the resistance of mice to radiation.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Gening</LastName><ForeName>L V</ForeName><Initials>LV</Initials><AffiliationInfo><Affiliation>Institute of Molecular Genetics, Russian Academy of Sciences, 123182 Moscow, Russia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Makarova</LastName><ForeName>A V</ForeName><Initials>AV</Initials></Author><Author ValidYN="Y"><LastName>Malashenko</LastName><ForeName>A M</ForeName><Initials>AM</Initials></Author><Author ValidYN="Y"><LastName>Tarantul</LastName><ForeName>V Z</ForeName><Initials>VZ</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Biochemistry (Mosc)</MedlineTA><NlmUniqueID>0376536</NlmUniqueID><ISSNLinking>0006-2979</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018389">Codon, Nonsense</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019384">Nucleic Acid Synthesis Inhibitors</NameOfSubstance></Chemical><Chemical><RegistryNumber>5Z93L87A1R</RegistryNumber><NameOfSubstance UI="D006147">Guanine</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.7.7.-</RegistryNumber><NameOfSubstance UI="C415422">DNA polymerase iota</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.7.7.7</RegistryNumber><NameOfSubstance UI="D004259">DNA-Directed DNA Polymerase</NameOfSubstance></Chemical><Chemical><RegistryNumber>QR26YLT7LT</RegistryNumber><NameOfSubstance UI="D013941">Thymine</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="ErratumIn"><RefSource>Biochemistry (Mosc). 2009 Jan;74(1):115</RefSource></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001921" MajorTopicYN="N">Brain</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020134" MajorTopicYN="N">Catalytic Domain</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018389" MajorTopicYN="N">Codon, Nonsense</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004261" MajorTopicYN="N">DNA Replication</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004259" MajorTopicYN="N">DNA-Directed DNA Polymerase</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D042822" MajorTopicYN="Y">Genomic Instability</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006147" MajorTopicYN="N">Guanine</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008807" MajorTopicYN="N">Mice, Inbred BALB C</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008809" MajorTopicYN="N">Mice, Inbred C3H</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008810" MajorTopicYN="N">Mice, Inbred C57BL</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019384" MajorTopicYN="N">Nucleic Acid Synthesis Inhibitors</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009928" MajorTopicYN="N">Organ Specificity</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013737" MajorTopicYN="N">Testis</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000528" MajorTopicYN="N">radiation effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013941" MajorTopicYN="N">Thymine</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2006</Year><Month>2</Month><Day>24</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2006</Year><Month>5</Month><Day>17</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2006</Year><Month>2</Month><Day>24</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16489919</ArticleId><ArticleId IdType="pii">BCM71020201</ArticleId><ArticleId IdType="doi">10.1134/s0006297906020064</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Russie</li></country><region><li>District fédéral central</li></region><settlement><li>Moscou</li></settlement></list><tree><noCountry><name sortKey="Makarova, A V" sort="Makarova, A V" uniqKey="Makarova A" first="A V" last="Makarova">A V Makarova</name><name sortKey="Malashenko, A M" sort="Malashenko, A M" uniqKey="Malashenko A" first="A M" last="Malashenko">A M Malashenko</name><name sortKey="Tarantul, V Z" sort="Tarantul, V Z" uniqKey="Tarantul V" first="V Z" last="Tarantul">V Z Tarantul</name></noCountry><country name="Russie"><region name="District fédéral central"><name sortKey="Gening, L V" sort="Gening, L V" uniqKey="Gening L" first="L V" last="Gening">L V Gening</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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